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e2 627 100  (R&D Systems)


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    Structured Review

    R&D Systems e2 627 100
    E2 627 100, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e2+627+100/Recombinant+Human%2FMouse%2FRat+UbcH5c%2FUBE2D3+Protein%2C+CF/pm41308642-530-59-61
    Average 95 stars, based on 55 article reviews
    e2 627 100 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Lysis:

    Article Title: A triple-action PROTAC for wild-type p53 cancer therapy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies His-Tag Cell Signaling Technology Cat# 2365S; RRID: AB_2115720 FLAG-Tag Cell Signaling Technology Cat# 2368S; RRID: AB_2217020 BRD4 Cell Signaling Technology Cat# 13440S; RRID: AB_2687578 p53 (DO-1) (Pantropic) Millipore Cat# OP43-100UG; RRID: AB_10682938 p21 Waf1/Cip1 (12S1) Cell Signaling Technology Cat# 2947S; RRID: AB_823586 β-actin Sigma Cat# A1978; RRID: AB_476692 HDM2 (Ab-1) (IF2) Millipore Cat# OP46-100UG; RRID: AB_564803 HDMX/MDM4 Fortis Life Sciences Cat# A300-287A; RRID: AB_263407 Goat anti-Mouse IgG - HRP BioRad Cat# 1706516; RRID: AB_2921252 Goat anti-Rabbit IgG - HRP Cell Signaling Technology Cat# 7074; RRID: AB_2099233 Goat anti-Rabbit IgG - Poly-HRP Invitrogen Cat# 32260; RRID: AB_1965959 BRD2 (D89B4) Cell Signaling Technology Cat# 5848; RRID: AB_10835146 BRD3 (E3D5N) Cell Signaling Technology Cat# 94302 Bacterial and virus strains pET28a vector Addgene Cat# 69864-3 pET15b vector Novagen Cat# 69661 BL21(DE3) E. coli NEB Cat# C2527H pNIC28Bsa4 vector Addgene Cat# 26103 Biological samples Pediatric Leukemia Blood (Primary T-ALL) DFCI Institutional Review Board Protocol #06-078 Chemicals, peptides, and recombinant proteins Ficoll-Paque PREMIUM Fisher Scientific Cat# 45-001-751 RPMI-1640 Gibco Cat# 21780-076 EMEM Gibco Cat# 11095080 DMEM Gibco Cat# 11965118 MEM Gibco Cat# 11095098 McCoy’s 5A (Modified) Medium Gibco Cat# 16600082 Ham’s F-12K (Kaighn’s) Medium Gibco Cat# 2127022 Fetal Bovine Serum Gemini Bio Cat# 100-106 Trypan Blue Gibco Cat# 15250-076 Pen-Strep Gibco Cat# 18140-122 (+)-JQ1 MedChemExpress Cat# HY-13030 (+)-JQ1-carboxylic acid MedChemExpress Cat# HY-78695 (Continued on next page) e1 Cell Reports Medicine 6, 102467, December 16, 2025 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER RG7388 MedChemExpress Cat# HY-15676 A1874 Selleckchem Cat# S8785 dBET6 MedChemExpress Cat# HY-112588 DMSO Sigma Cat# D2650-100ML 1M HEPES, pH 7.5 Gibco Cat# 15630-080 TCEP Thermo Cat# 77720 RIPA lysis buffer Sigma Cat# 20-188 NaCl Fisher Scientific Cat# BP358-212 Human GST-HDM2 R&D Systems Cat# E3-202-050 UBE1 R&D Systems Cat# E− 305-025 UbcH5c/UBE2D3 R&D Systems Cat# E2-627-100 Mg-ATP R&D Systems Cat# B-20 FLAG-p53 BPS Bioscience Cat# 100412 6xHis-BRD4 (BD1+BD2) BPS Bioscience Cat# 31045 4-12% Bis-Tris Gels Invitrogen Cat# NP0335BOX 3-8% Tris-Acetate Gels Invitrogen Cat# EA0375BOX NuPAGE LDS Sample Buffer (4×) Thermo Cat# NP0008 Glycerol Sigma Cat# G9012-1L DTT Gold Biotechnology Cat# 27565-41-9 Isopropyl β-D-1-thiogalactopyranoside Gold Biotechnology Cat# 367-93-1 Protease inhibitor cocktail Sigma Cat# 11873580001 PhosSTOP phosphatase inhibitor Sigma Cat# 4906845001 Urea Sigma Cat# U53758 Iodoacetamide Pierce Cat# A39271 Chloroform Sigma Cat# CX1055 Methanol Fisher Scientific Cat# A412-4 LysC Wako Cat# 129-02541 Trypsin Pierce Cat# 90305 Hydroxylamine Sigma Cat# 467804 TMTpro 18-plex reagents Thermo Cat# A52045 Sep-Pal C18 Waters Cat# WAT020515 Imidazole Sigma Cat# I202 BME Thermo Cat# 21985023 IGEPAL Thermo Cat# J61055.AE PMSF Thermo Cat# 36978 Ni-NTA beads Qiagen Cat# 30210 Bortezomib Sigma Cat# 5043140001 Cycloheximide Thermo Cat# J66004.XF Dynabeads Epoxy 270 Thermo Cat# 14301 EDTA Sigma Cat# E4884 EPPS Thermo Cat# 561476.AK Critical commercial assays CellTiter-Glo Promega Cat# G7571 iBlot2 NC ministacks Invitrogen Cat# 1323002 Caspase-Glo® 3/7 Assay System Promega Cat# G8090 Senescence β-Galactosidase Activity Assay Kit Cell Signaling Technology Cat# 23833 MycoAlert mycoplasma detection kit Lonza Biologics Cat# LT07-318 BCA protein assay kit Thermo Cat# 23227 Western blot detection kit Cytiva Cat# 45-002-401 (Continued on next page) Cell Reports Medicine 6, 102467, December 16, 2025 e2 OPEN ACCESS .. Cultured cell lines were purchased from and authenticated by ATCC.

    Protease Inhibitor:

    Article Title: A triple-action PROTAC for wild-type p53 cancer therapy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies His-Tag Cell Signaling Technology Cat# 2365S; RRID: AB_2115720 FLAG-Tag Cell Signaling Technology Cat# 2368S; RRID: AB_2217020 BRD4 Cell Signaling Technology Cat# 13440S; RRID: AB_2687578 p53 (DO-1) (Pantropic) Millipore Cat# OP43-100UG; RRID: AB_10682938 p21 Waf1/Cip1 (12S1) Cell Signaling Technology Cat# 2947S; RRID: AB_823586 β-actin Sigma Cat# A1978; RRID: AB_476692 HDM2 (Ab-1) (IF2) Millipore Cat# OP46-100UG; RRID: AB_564803 HDMX/MDM4 Fortis Life Sciences Cat# A300-287A; RRID: AB_263407 Goat anti-Mouse IgG - HRP BioRad Cat# 1706516; RRID: AB_2921252 Goat anti-Rabbit IgG - HRP Cell Signaling Technology Cat# 7074; RRID: AB_2099233 Goat anti-Rabbit IgG - Poly-HRP Invitrogen Cat# 32260; RRID: AB_1965959 BRD2 (D89B4) Cell Signaling Technology Cat# 5848; RRID: AB_10835146 BRD3 (E3D5N) Cell Signaling Technology Cat# 94302 Bacterial and virus strains pET28a vector Addgene Cat# 69864-3 pET15b vector Novagen Cat# 69661 BL21(DE3) E. coli NEB Cat# C2527H pNIC28Bsa4 vector Addgene Cat# 26103 Biological samples Pediatric Leukemia Blood (Primary T-ALL) DFCI Institutional Review Board Protocol #06-078 Chemicals, peptides, and recombinant proteins Ficoll-Paque PREMIUM Fisher Scientific Cat# 45-001-751 RPMI-1640 Gibco Cat# 21780-076 EMEM Gibco Cat# 11095080 DMEM Gibco Cat# 11965118 MEM Gibco Cat# 11095098 McCoy’s 5A (Modified) Medium Gibco Cat# 16600082 Ham’s F-12K (Kaighn’s) Medium Gibco Cat# 2127022 Fetal Bovine Serum Gemini Bio Cat# 100-106 Trypan Blue Gibco Cat# 15250-076 Pen-Strep Gibco Cat# 18140-122 (+)-JQ1 MedChemExpress Cat# HY-13030 (+)-JQ1-carboxylic acid MedChemExpress Cat# HY-78695 (Continued on next page) e1 Cell Reports Medicine 6, 102467, December 16, 2025 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER RG7388 MedChemExpress Cat# HY-15676 A1874 Selleckchem Cat# S8785 dBET6 MedChemExpress Cat# HY-112588 DMSO Sigma Cat# D2650-100ML 1M HEPES, pH 7.5 Gibco Cat# 15630-080 TCEP Thermo Cat# 77720 RIPA lysis buffer Sigma Cat# 20-188 NaCl Fisher Scientific Cat# BP358-212 Human GST-HDM2 R&D Systems Cat# E3-202-050 UBE1 R&D Systems Cat# E− 305-025 UbcH5c/UBE2D3 R&D Systems Cat# E2-627-100 Mg-ATP R&D Systems Cat# B-20 FLAG-p53 BPS Bioscience Cat# 100412 6xHis-BRD4 (BD1+BD2) BPS Bioscience Cat# 31045 4-12% Bis-Tris Gels Invitrogen Cat# NP0335BOX 3-8% Tris-Acetate Gels Invitrogen Cat# EA0375BOX NuPAGE LDS Sample Buffer (4×) Thermo Cat# NP0008 Glycerol Sigma Cat# G9012-1L DTT Gold Biotechnology Cat# 27565-41-9 Isopropyl β-D-1-thiogalactopyranoside Gold Biotechnology Cat# 367-93-1 Protease inhibitor cocktail Sigma Cat# 11873580001 PhosSTOP phosphatase inhibitor Sigma Cat# 4906845001 Urea Sigma Cat# U53758 Iodoacetamide Pierce Cat# A39271 Chloroform Sigma Cat# CX1055 Methanol Fisher Scientific Cat# A412-4 LysC Wako Cat# 129-02541 Trypsin Pierce Cat# 90305 Hydroxylamine Sigma Cat# 467804 TMTpro 18-plex reagents Thermo Cat# A52045 Sep-Pal C18 Waters Cat# WAT020515 Imidazole Sigma Cat# I202 BME Thermo Cat# 21985023 IGEPAL Thermo Cat# J61055.AE PMSF Thermo Cat# 36978 Ni-NTA beads Qiagen Cat# 30210 Bortezomib Sigma Cat# 5043140001 Cycloheximide Thermo Cat# J66004.XF Dynabeads Epoxy 270 Thermo Cat# 14301 EDTA Sigma Cat# E4884 EPPS Thermo Cat# 561476.AK Critical commercial assays CellTiter-Glo Promega Cat# G7571 iBlot2 NC ministacks Invitrogen Cat# 1323002 Caspase-Glo® 3/7 Assay System Promega Cat# G8090 Senescence β-Galactosidase Activity Assay Kit Cell Signaling Technology Cat# 23833 MycoAlert mycoplasma detection kit Lonza Biologics Cat# LT07-318 BCA protein assay kit Thermo Cat# 23227 Western blot detection kit Cytiva Cat# 45-002-401 (Continued on next page) Cell Reports Medicine 6, 102467, December 16, 2025 e2 OPEN ACCESS .. Cultured cell lines were purchased from and authenticated by ATCC.

    Caspase-Glo Assay:

    Article Title: A triple-action PROTAC for wild-type p53 cancer therapy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies His-Tag Cell Signaling Technology Cat# 2365S; RRID: AB_2115720 FLAG-Tag Cell Signaling Technology Cat# 2368S; RRID: AB_2217020 BRD4 Cell Signaling Technology Cat# 13440S; RRID: AB_2687578 p53 (DO-1) (Pantropic) Millipore Cat# OP43-100UG; RRID: AB_10682938 p21 Waf1/Cip1 (12S1) Cell Signaling Technology Cat# 2947S; RRID: AB_823586 β-actin Sigma Cat# A1978; RRID: AB_476692 HDM2 (Ab-1) (IF2) Millipore Cat# OP46-100UG; RRID: AB_564803 HDMX/MDM4 Fortis Life Sciences Cat# A300-287A; RRID: AB_263407 Goat anti-Mouse IgG - HRP BioRad Cat# 1706516; RRID: AB_2921252 Goat anti-Rabbit IgG - HRP Cell Signaling Technology Cat# 7074; RRID: AB_2099233 Goat anti-Rabbit IgG - Poly-HRP Invitrogen Cat# 32260; RRID: AB_1965959 BRD2 (D89B4) Cell Signaling Technology Cat# 5848; RRID: AB_10835146 BRD3 (E3D5N) Cell Signaling Technology Cat# 94302 Bacterial and virus strains pET28a vector Addgene Cat# 69864-3 pET15b vector Novagen Cat# 69661 BL21(DE3) E. coli NEB Cat# C2527H pNIC28Bsa4 vector Addgene Cat# 26103 Biological samples Pediatric Leukemia Blood (Primary T-ALL) DFCI Institutional Review Board Protocol #06-078 Chemicals, peptides, and recombinant proteins Ficoll-Paque PREMIUM Fisher Scientific Cat# 45-001-751 RPMI-1640 Gibco Cat# 21780-076 EMEM Gibco Cat# 11095080 DMEM Gibco Cat# 11965118 MEM Gibco Cat# 11095098 McCoy’s 5A (Modified) Medium Gibco Cat# 16600082 Ham’s F-12K (Kaighn’s) Medium Gibco Cat# 2127022 Fetal Bovine Serum Gemini Bio Cat# 100-106 Trypan Blue Gibco Cat# 15250-076 Pen-Strep Gibco Cat# 18140-122 (+)-JQ1 MedChemExpress Cat# HY-13030 (+)-JQ1-carboxylic acid MedChemExpress Cat# HY-78695 (Continued on next page) e1 Cell Reports Medicine 6, 102467, December 16, 2025 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER RG7388 MedChemExpress Cat# HY-15676 A1874 Selleckchem Cat# S8785 dBET6 MedChemExpress Cat# HY-112588 DMSO Sigma Cat# D2650-100ML 1M HEPES, pH 7.5 Gibco Cat# 15630-080 TCEP Thermo Cat# 77720 RIPA lysis buffer Sigma Cat# 20-188 NaCl Fisher Scientific Cat# BP358-212 Human GST-HDM2 R&D Systems Cat# E3-202-050 UBE1 R&D Systems Cat# E− 305-025 UbcH5c/UBE2D3 R&D Systems Cat# E2-627-100 Mg-ATP R&D Systems Cat# B-20 FLAG-p53 BPS Bioscience Cat# 100412 6xHis-BRD4 (BD1+BD2) BPS Bioscience Cat# 31045 4-12% Bis-Tris Gels Invitrogen Cat# NP0335BOX 3-8% Tris-Acetate Gels Invitrogen Cat# EA0375BOX NuPAGE LDS Sample Buffer (4×) Thermo Cat# NP0008 Glycerol Sigma Cat# G9012-1L DTT Gold Biotechnology Cat# 27565-41-9 Isopropyl β-D-1-thiogalactopyranoside Gold Biotechnology Cat# 367-93-1 Protease inhibitor cocktail Sigma Cat# 11873580001 PhosSTOP phosphatase inhibitor Sigma Cat# 4906845001 Urea Sigma Cat# U53758 Iodoacetamide Pierce Cat# A39271 Chloroform Sigma Cat# CX1055 Methanol Fisher Scientific Cat# A412-4 LysC Wako Cat# 129-02541 Trypsin Pierce Cat# 90305 Hydroxylamine Sigma Cat# 467804 TMTpro 18-plex reagents Thermo Cat# A52045 Sep-Pal C18 Waters Cat# WAT020515 Imidazole Sigma Cat# I202 BME Thermo Cat# 21985023 IGEPAL Thermo Cat# J61055.AE PMSF Thermo Cat# 36978 Ni-NTA beads Qiagen Cat# 30210 Bortezomib Sigma Cat# 5043140001 Cycloheximide Thermo Cat# J66004.XF Dynabeads Epoxy 270 Thermo Cat# 14301 EDTA Sigma Cat# E4884 EPPS Thermo Cat# 561476.AK Critical commercial assays CellTiter-Glo Promega Cat# G7571 iBlot2 NC ministacks Invitrogen Cat# 1323002 Caspase-Glo® 3/7 Assay System Promega Cat# G8090 Senescence β-Galactosidase Activity Assay Kit Cell Signaling Technology Cat# 23833 MycoAlert mycoplasma detection kit Lonza Biologics Cat# LT07-318 BCA protein assay kit Thermo Cat# 23227 Western blot detection kit Cytiva Cat# 45-002-401 (Continued on next page) Cell Reports Medicine 6, 102467, December 16, 2025 e2 OPEN ACCESS .. Cultured cell lines were purchased from and authenticated by ATCC.

    Activity Assay:

    Article Title: A triple-action PROTAC for wild-type p53 cancer therapy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies His-Tag Cell Signaling Technology Cat# 2365S; RRID: AB_2115720 FLAG-Tag Cell Signaling Technology Cat# 2368S; RRID: AB_2217020 BRD4 Cell Signaling Technology Cat# 13440S; RRID: AB_2687578 p53 (DO-1) (Pantropic) Millipore Cat# OP43-100UG; RRID: AB_10682938 p21 Waf1/Cip1 (12S1) Cell Signaling Technology Cat# 2947S; RRID: AB_823586 β-actin Sigma Cat# A1978; RRID: AB_476692 HDM2 (Ab-1) (IF2) Millipore Cat# OP46-100UG; RRID: AB_564803 HDMX/MDM4 Fortis Life Sciences Cat# A300-287A; RRID: AB_263407 Goat anti-Mouse IgG - HRP BioRad Cat# 1706516; RRID: AB_2921252 Goat anti-Rabbit IgG - HRP Cell Signaling Technology Cat# 7074; RRID: AB_2099233 Goat anti-Rabbit IgG - Poly-HRP Invitrogen Cat# 32260; RRID: AB_1965959 BRD2 (D89B4) Cell Signaling Technology Cat# 5848; RRID: AB_10835146 BRD3 (E3D5N) Cell Signaling Technology Cat# 94302 Bacterial and virus strains pET28a vector Addgene Cat# 69864-3 pET15b vector Novagen Cat# 69661 BL21(DE3) E. coli NEB Cat# C2527H pNIC28Bsa4 vector Addgene Cat# 26103 Biological samples Pediatric Leukemia Blood (Primary T-ALL) DFCI Institutional Review Board Protocol #06-078 Chemicals, peptides, and recombinant proteins Ficoll-Paque PREMIUM Fisher Scientific Cat# 45-001-751 RPMI-1640 Gibco Cat# 21780-076 EMEM Gibco Cat# 11095080 DMEM Gibco Cat# 11965118 MEM Gibco Cat# 11095098 McCoy’s 5A (Modified) Medium Gibco Cat# 16600082 Ham’s F-12K (Kaighn’s) Medium Gibco Cat# 2127022 Fetal Bovine Serum Gemini Bio Cat# 100-106 Trypan Blue Gibco Cat# 15250-076 Pen-Strep Gibco Cat# 18140-122 (+)-JQ1 MedChemExpress Cat# HY-13030 (+)-JQ1-carboxylic acid MedChemExpress Cat# HY-78695 (Continued on next page) e1 Cell Reports Medicine 6, 102467, December 16, 2025 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER RG7388 MedChemExpress Cat# HY-15676 A1874 Selleckchem Cat# S8785 dBET6 MedChemExpress Cat# HY-112588 DMSO Sigma Cat# D2650-100ML 1M HEPES, pH 7.5 Gibco Cat# 15630-080 TCEP Thermo Cat# 77720 RIPA lysis buffer Sigma Cat# 20-188 NaCl Fisher Scientific Cat# BP358-212 Human GST-HDM2 R&D Systems Cat# E3-202-050 UBE1 R&D Systems Cat# E− 305-025 UbcH5c/UBE2D3 R&D Systems Cat# E2-627-100 Mg-ATP R&D Systems Cat# B-20 FLAG-p53 BPS Bioscience Cat# 100412 6xHis-BRD4 (BD1+BD2) BPS Bioscience Cat# 31045 4-12% Bis-Tris Gels Invitrogen Cat# NP0335BOX 3-8% Tris-Acetate Gels Invitrogen Cat# EA0375BOX NuPAGE LDS Sample Buffer (4×) Thermo Cat# NP0008 Glycerol Sigma Cat# G9012-1L DTT Gold Biotechnology Cat# 27565-41-9 Isopropyl β-D-1-thiogalactopyranoside Gold Biotechnology Cat# 367-93-1 Protease inhibitor cocktail Sigma Cat# 11873580001 PhosSTOP phosphatase inhibitor Sigma Cat# 4906845001 Urea Sigma Cat# U53758 Iodoacetamide Pierce Cat# A39271 Chloroform Sigma Cat# CX1055 Methanol Fisher Scientific Cat# A412-4 LysC Wako Cat# 129-02541 Trypsin Pierce Cat# 90305 Hydroxylamine Sigma Cat# 467804 TMTpro 18-plex reagents Thermo Cat# A52045 Sep-Pal C18 Waters Cat# WAT020515 Imidazole Sigma Cat# I202 BME Thermo Cat# 21985023 IGEPAL Thermo Cat# J61055.AE PMSF Thermo Cat# 36978 Ni-NTA beads Qiagen Cat# 30210 Bortezomib Sigma Cat# 5043140001 Cycloheximide Thermo Cat# J66004.XF Dynabeads Epoxy 270 Thermo Cat# 14301 EDTA Sigma Cat# E4884 EPPS Thermo Cat# 561476.AK Critical commercial assays CellTiter-Glo Promega Cat# G7571 iBlot2 NC ministacks Invitrogen Cat# 1323002 Caspase-Glo® 3/7 Assay System Promega Cat# G8090 Senescence β-Galactosidase Activity Assay Kit Cell Signaling Technology Cat# 23833 MycoAlert mycoplasma detection kit Lonza Biologics Cat# LT07-318 BCA protein assay kit Thermo Cat# 23227 Western blot detection kit Cytiva Cat# 45-002-401 (Continued on next page) Cell Reports Medicine 6, 102467, December 16, 2025 e2 OPEN ACCESS .. Cultured cell lines were purchased from and authenticated by ATCC.

    Bicinchoninic Acid Protein Assay:

    Article Title: A triple-action PROTAC for wild-type p53 cancer therapy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies His-Tag Cell Signaling Technology Cat# 2365S; RRID: AB_2115720 FLAG-Tag Cell Signaling Technology Cat# 2368S; RRID: AB_2217020 BRD4 Cell Signaling Technology Cat# 13440S; RRID: AB_2687578 p53 (DO-1) (Pantropic) Millipore Cat# OP43-100UG; RRID: AB_10682938 p21 Waf1/Cip1 (12S1) Cell Signaling Technology Cat# 2947S; RRID: AB_823586 β-actin Sigma Cat# A1978; RRID: AB_476692 HDM2 (Ab-1) (IF2) Millipore Cat# OP46-100UG; RRID: AB_564803 HDMX/MDM4 Fortis Life Sciences Cat# A300-287A; RRID: AB_263407 Goat anti-Mouse IgG - HRP BioRad Cat# 1706516; RRID: AB_2921252 Goat anti-Rabbit IgG - HRP Cell Signaling Technology Cat# 7074; RRID: AB_2099233 Goat anti-Rabbit IgG - Poly-HRP Invitrogen Cat# 32260; RRID: AB_1965959 BRD2 (D89B4) Cell Signaling Technology Cat# 5848; RRID: AB_10835146 BRD3 (E3D5N) Cell Signaling Technology Cat# 94302 Bacterial and virus strains pET28a vector Addgene Cat# 69864-3 pET15b vector Novagen Cat# 69661 BL21(DE3) E. coli NEB Cat# C2527H pNIC28Bsa4 vector Addgene Cat# 26103 Biological samples Pediatric Leukemia Blood (Primary T-ALL) DFCI Institutional Review Board Protocol #06-078 Chemicals, peptides, and recombinant proteins Ficoll-Paque PREMIUM Fisher Scientific Cat# 45-001-751 RPMI-1640 Gibco Cat# 21780-076 EMEM Gibco Cat# 11095080 DMEM Gibco Cat# 11965118 MEM Gibco Cat# 11095098 McCoy’s 5A (Modified) Medium Gibco Cat# 16600082 Ham’s F-12K (Kaighn’s) Medium Gibco Cat# 2127022 Fetal Bovine Serum Gemini Bio Cat# 100-106 Trypan Blue Gibco Cat# 15250-076 Pen-Strep Gibco Cat# 18140-122 (+)-JQ1 MedChemExpress Cat# HY-13030 (+)-JQ1-carboxylic acid MedChemExpress Cat# HY-78695 (Continued on next page) e1 Cell Reports Medicine 6, 102467, December 16, 2025 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER RG7388 MedChemExpress Cat# HY-15676 A1874 Selleckchem Cat# S8785 dBET6 MedChemExpress Cat# HY-112588 DMSO Sigma Cat# D2650-100ML 1M HEPES, pH 7.5 Gibco Cat# 15630-080 TCEP Thermo Cat# 77720 RIPA lysis buffer Sigma Cat# 20-188 NaCl Fisher Scientific Cat# BP358-212 Human GST-HDM2 R&D Systems Cat# E3-202-050 UBE1 R&D Systems Cat# E− 305-025 UbcH5c/UBE2D3 R&D Systems Cat# E2-627-100 Mg-ATP R&D Systems Cat# B-20 FLAG-p53 BPS Bioscience Cat# 100412 6xHis-BRD4 (BD1+BD2) BPS Bioscience Cat# 31045 4-12% Bis-Tris Gels Invitrogen Cat# NP0335BOX 3-8% Tris-Acetate Gels Invitrogen Cat# EA0375BOX NuPAGE LDS Sample Buffer (4×) Thermo Cat# NP0008 Glycerol Sigma Cat# G9012-1L DTT Gold Biotechnology Cat# 27565-41-9 Isopropyl β-D-1-thiogalactopyranoside Gold Biotechnology Cat# 367-93-1 Protease inhibitor cocktail Sigma Cat# 11873580001 PhosSTOP phosphatase inhibitor Sigma Cat# 4906845001 Urea Sigma Cat# U53758 Iodoacetamide Pierce Cat# A39271 Chloroform Sigma Cat# CX1055 Methanol Fisher Scientific Cat# A412-4 LysC Wako Cat# 129-02541 Trypsin Pierce Cat# 90305 Hydroxylamine Sigma Cat# 467804 TMTpro 18-plex reagents Thermo Cat# A52045 Sep-Pal C18 Waters Cat# WAT020515 Imidazole Sigma Cat# I202 BME Thermo Cat# 21985023 IGEPAL Thermo Cat# J61055.AE PMSF Thermo Cat# 36978 Ni-NTA beads Qiagen Cat# 30210 Bortezomib Sigma Cat# 5043140001 Cycloheximide Thermo Cat# J66004.XF Dynabeads Epoxy 270 Thermo Cat# 14301 EDTA Sigma Cat# E4884 EPPS Thermo Cat# 561476.AK Critical commercial assays CellTiter-Glo Promega Cat# G7571 iBlot2 NC ministacks Invitrogen Cat# 1323002 Caspase-Glo® 3/7 Assay System Promega Cat# G8090 Senescence β-Galactosidase Activity Assay Kit Cell Signaling Technology Cat# 23833 MycoAlert mycoplasma detection kit Lonza Biologics Cat# LT07-318 BCA protein assay kit Thermo Cat# 23227 Western blot detection kit Cytiva Cat# 45-002-401 (Continued on next page) Cell Reports Medicine 6, 102467, December 16, 2025 e2 OPEN ACCESS .. Cultured cell lines were purchased from and authenticated by ATCC.

    Western Blot:

    Article Title: A triple-action PROTAC for wild-type p53 cancer therapy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies His-Tag Cell Signaling Technology Cat# 2365S; RRID: AB_2115720 FLAG-Tag Cell Signaling Technology Cat# 2368S; RRID: AB_2217020 BRD4 Cell Signaling Technology Cat# 13440S; RRID: AB_2687578 p53 (DO-1) (Pantropic) Millipore Cat# OP43-100UG; RRID: AB_10682938 p21 Waf1/Cip1 (12S1) Cell Signaling Technology Cat# 2947S; RRID: AB_823586 β-actin Sigma Cat# A1978; RRID: AB_476692 HDM2 (Ab-1) (IF2) Millipore Cat# OP46-100UG; RRID: AB_564803 HDMX/MDM4 Fortis Life Sciences Cat# A300-287A; RRID: AB_263407 Goat anti-Mouse IgG - HRP BioRad Cat# 1706516; RRID: AB_2921252 Goat anti-Rabbit IgG - HRP Cell Signaling Technology Cat# 7074; RRID: AB_2099233 Goat anti-Rabbit IgG - Poly-HRP Invitrogen Cat# 32260; RRID: AB_1965959 BRD2 (D89B4) Cell Signaling Technology Cat# 5848; RRID: AB_10835146 BRD3 (E3D5N) Cell Signaling Technology Cat# 94302 Bacterial and virus strains pET28a vector Addgene Cat# 69864-3 pET15b vector Novagen Cat# 69661 BL21(DE3) E. coli NEB Cat# C2527H pNIC28Bsa4 vector Addgene Cat# 26103 Biological samples Pediatric Leukemia Blood (Primary T-ALL) DFCI Institutional Review Board Protocol #06-078 Chemicals, peptides, and recombinant proteins Ficoll-Paque PREMIUM Fisher Scientific Cat# 45-001-751 RPMI-1640 Gibco Cat# 21780-076 EMEM Gibco Cat# 11095080 DMEM Gibco Cat# 11965118 MEM Gibco Cat# 11095098 McCoy’s 5A (Modified) Medium Gibco Cat# 16600082 Ham’s F-12K (Kaighn’s) Medium Gibco Cat# 2127022 Fetal Bovine Serum Gemini Bio Cat# 100-106 Trypan Blue Gibco Cat# 15250-076 Pen-Strep Gibco Cat# 18140-122 (+)-JQ1 MedChemExpress Cat# HY-13030 (+)-JQ1-carboxylic acid MedChemExpress Cat# HY-78695 (Continued on next page) e1 Cell Reports Medicine 6, 102467, December 16, 2025 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER RG7388 MedChemExpress Cat# HY-15676 A1874 Selleckchem Cat# S8785 dBET6 MedChemExpress Cat# HY-112588 DMSO Sigma Cat# D2650-100ML 1M HEPES, pH 7.5 Gibco Cat# 15630-080 TCEP Thermo Cat# 77720 RIPA lysis buffer Sigma Cat# 20-188 NaCl Fisher Scientific Cat# BP358-212 Human GST-HDM2 R&D Systems Cat# E3-202-050 UBE1 R&D Systems Cat# E− 305-025 UbcH5c/UBE2D3 R&D Systems Cat# E2-627-100 Mg-ATP R&D Systems Cat# B-20 FLAG-p53 BPS Bioscience Cat# 100412 6xHis-BRD4 (BD1+BD2) BPS Bioscience Cat# 31045 4-12% Bis-Tris Gels Invitrogen Cat# NP0335BOX 3-8% Tris-Acetate Gels Invitrogen Cat# EA0375BOX NuPAGE LDS Sample Buffer (4×) Thermo Cat# NP0008 Glycerol Sigma Cat# G9012-1L DTT Gold Biotechnology Cat# 27565-41-9 Isopropyl β-D-1-thiogalactopyranoside Gold Biotechnology Cat# 367-93-1 Protease inhibitor cocktail Sigma Cat# 11873580001 PhosSTOP phosphatase inhibitor Sigma Cat# 4906845001 Urea Sigma Cat# U53758 Iodoacetamide Pierce Cat# A39271 Chloroform Sigma Cat# CX1055 Methanol Fisher Scientific Cat# A412-4 LysC Wako Cat# 129-02541 Trypsin Pierce Cat# 90305 Hydroxylamine Sigma Cat# 467804 TMTpro 18-plex reagents Thermo Cat# A52045 Sep-Pal C18 Waters Cat# WAT020515 Imidazole Sigma Cat# I202 BME Thermo Cat# 21985023 IGEPAL Thermo Cat# J61055.AE PMSF Thermo Cat# 36978 Ni-NTA beads Qiagen Cat# 30210 Bortezomib Sigma Cat# 5043140001 Cycloheximide Thermo Cat# J66004.XF Dynabeads Epoxy 270 Thermo Cat# 14301 EDTA Sigma Cat# E4884 EPPS Thermo Cat# 561476.AK Critical commercial assays CellTiter-Glo Promega Cat# G7571 iBlot2 NC ministacks Invitrogen Cat# 1323002 Caspase-Glo® 3/7 Assay System Promega Cat# G8090 Senescence β-Galactosidase Activity Assay Kit Cell Signaling Technology Cat# 23833 MycoAlert mycoplasma detection kit Lonza Biologics Cat# LT07-318 BCA protein assay kit Thermo Cat# 23227 Western blot detection kit Cytiva Cat# 45-002-401 (Continued on next page) Cell Reports Medicine 6, 102467, December 16, 2025 e2 OPEN ACCESS .. Cultured cell lines were purchased from and authenticated by ATCC.



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    e2 627 100  (R&D Systems)
    95
    R&D Systems e2 627 100
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